Enzyme-linked immunosorbent assay for detection of Streptococcus pneumoniae antigen
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چکیده
منابع مشابه
Enzyme-linked immunosorbent assay for detection of Streptococcus pneumoniae antigen.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Streptococcus pneumoniae polysaccharide antigen in cerebrospinal fluid and serum. Sensitivity and specificity were determined for purified antigen preparations. Specificity was also evaluated in the rabbit meningitis model, and the sensitivity was compared to counterimmunoelectrophoresis, using the infected rabbits'...
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An indirect sandwich enzyme-linked immunosorbent assay for the detection of the polysaccharide antigen of type 3 pneumococcus (SSS-III) is reported. Polystyrene balls with attached anti-SSS-III antibody serve as the solid phase, and antigen is detected using an alkaline phosphatase-labeled antiglobulin conjugate. The reaction is quantitated by spectrophotometry. Concentrations of antigen are es...
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Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...
متن کاملEnzyme-linked immunosorbent assay for detection of Mycoplasma pneumoniae antibodies.
The enzyme-linked immunosorbent assay (ELISA) described by Engvall and Perlmann has been used for the detection of antibodies against Mycoplasma pneumoniae. These organisms, grown in flat-bottom wells of microtiter plates, were used as antigen. Preliminary results suggest that ELISA is specific and that its sensitivity is somewhere in between those of the metabolic inhibition and radioimmunopre...
متن کاملDiagnosis of Streptococcus pneumoniae pneumonia by quantitative enzyme linked immunosorbent assay of C-polysaccharide antigen.
AIMS To evaluate the use of a quantitative enzyme linked immunosorbent assay (ELISA) detecting C-polysaccharide (PnC) antigen in sputum for the diagnosis of Streptococcus pneumoniae infection. METHODS Specimens of sputum from 60 patients with acute community and hospital acquired pneumonia and infective exacerbations of obstructive airways disease were examined by semiquantitative culture and...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1979
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.10.3.339-342.1979